The aim of the FP6 project carcinoGENOMICS is to develop in vitro methods for assessing the carcinogenic potential of compounds, as an alternative to current rodent bioassays for genotoxicity and carcinogenicity. The major goal is to develop a battery of mechanism-based in vitro tests accounting for various modes of carcinogenic action. These tests will be designed to cover major target organs for carcinogenic action e.g. the liver, the lung and the kidney. The novel assays will be based on the application of "omics" technologies (i.e. genome-wide transcriptomics as well as metabonomics) to robust in vitro systems (rat/human), thereby also exploring stem cell technology, to generate "omic" responses from a well-defined set of model compounds causing genotoxicity and carcinogenicity. Phenotypic markers for genotoxic and carcinogenic events will be assessed for the purpose of anchoring gene expression modulations, metabolic profiles and mechanistic pathways. Through extensive biostatistics, literature mining, and analysis of molecular-expression datasets, differential genetic pathways will be identified capable of predicting mechanisms of chemical carcinogenesis in vivo.
Furthermore, generated transcriptomic and metabonomic data will be integrated into a holistic understanding of systems biology, and applied to build an iterative in silico model of chemical carcinogenesis. Subsequently, predictive genetic pathways will be used as the scientific basis to develop high throughput technology for accelerating analysis of genomics responses in vitro, indicative for human carcinogenic risk, by a factor of 100.
It is expected that the outcome of this project will generate a platform enabling the investigation of large numbers of compounds for their genotoxic and carcinogenic potential. This will contribute to speeding the identification of potential harmful substances to man, while lowering costs and reducing animal tests.
Carcinogenomics FINAL brochure is now available for download ! Click here to download:
The Seurat-1 Annual reports aim to inform policy makers about scientific progress relevant to the implementation of European Directives and Regulations and about essential gaps in knowledge corresponding to research needs. They will in particular contain detailed information about:
· the scientific progress,
· the strategic development,
· the evolution of the legislative and regulatory context,
in the field of repeated dose systemic toxicity testing.
Now available for download. Click here to download: